Analysis of anti-diabetic properties of Phyllanthus urinaria by docking studies

The objective of this research was to analyze the anti-diabetic properties of the bioactive compounds extracted from P. urinaria using molecular docking servers. Two extracts were prepared using distilled water and chloroform as solvents by optimized maceration procedure. Determination of phytochemical constituents of these extracts was then carried out by using standard set of protocols. GC-MS analysis was carried out to obtain the physical properties and the structural analogs of the phytochemicals. Docking of the respective phytochemicals with TXNIP using metformin as the reference drug was performed using Patch dock. Upon phytochemical screening it was found that both the chloroform and aqueous extract contain bioactive compounds. Comparatively the aqueous extract was found to be richer in phytochemicals than the chloroform extract. The GC-MS study provided the structural analog and the physical properties of six phytochemicals present in the chloroform extract. Docking study confirmed the fact that five out of the six phytochemicals have better binding efficiency to TXNIP as compared to the conventional drug Metformin. The phytochemicals so obtained have better efficiency in lowering blood glucose level as compared to metformin. The present study confirms the fact that the bioactive so obtained possess efficient anti-diabetic properties. The molecular docking study confirms the fact that the phytochemicals have better efficiency in lowering blood glucose level. Hence the phytochemicals from P. urinaria can be a substitute for the more toxic drug Metformin for diabetes treatment.